Micro Baterial Morphology Lab

Bacterial Morphology Part 1 Viewing Prepared Slides of prevalent Bacterial Shapes Familiarize yourself with from each one morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2 Disinfecting Your Area to Use get laid Organisms Part 3 Viewing Live Organisms Wet Mount Preparation there was several amoeba regulate cells that varied in size. There were five somewhat darker areas that were circular in grade. There were also three large, oddly shaped areas that had very apparent edges. Part 4 Direct Staining Slide One There were two clusters that were fairly easy to recognize.All of the cells were coccus. Some of the cells were large while others were a good deal nonexistent. Slide Two There were different layers of cells. The cells were rectangular in shape and varied in size. A nucleus was visible in each cell. Slide Three This slide was a mixture of different shapes. postal code was recognizable. Part 5 In sway Staini ng Chains of both cocci and bracillus cells were both visible and identifiable. The chains varied in length. The hardihood and yeast denigrate was clearer. The same shapes were seen as before just with sharper outlines.The cells were frequently easier to see with more detail. Questions A. What are the advantages of using bleach as a disinfectant? The disadvantages? The advantages of using 70% alcohol? The disadvantages? B. List three reasons why you might choose to stain a particular slide earlier than view it as a wet mount. C. Define the following toll Chromophore Acidic Dye Basic Dye D. What is the difference between direct and indirect staining? E. What is heat altering? F. Why is it necessary to tell that your specimens are completely air dried prior to heat fixing? G.Describe what you sight in your plaque smear wet mount, direct varnished slide, and indirectly varnished slide. What were the similarities? What were the differences? H. Describe what you observed in your cheek smear wet mount, direct stained slide, and indirectly stained slide. What were the similarities? What were the differences? I. Describe what you observed in your yeast wet mount, direct stained slide, and indirectly stained slide. What were the similarities? What were the differences? J. Were the cell types the same in all three specimen sets yeast, plaque, and cheek? How were they similar? How were they different?