Spectrophotometer

Determination of the unknown tightness and absorbance of Cobalt Chloride solution by operating the Spectrophotometer Van Nguyen BIOSC 48, Sec 21453 Date September 11, 2012 universe We are going to use spectrophotometric techniques by using a spectrophotometer, which assesss the absorbance by a solution of light of specific wavelength. Also, it allows us to determine the unknown concentration of solution. To do this, we will arise the summation of solution in cuvettes that are placed in the spectrophotometer. Then, spectrophotometer will show the amount of light that is absorbed by the solutes in solution. Spectrophotometry takes advantage of the dual nature of light and barely work with visible light. In this experiment, I was introduced to the spectrophotometry as yawl up as how it is used to measure the concentration of step up CoCl2. By seeing the absorbance of CoCl2 at different wavelengths and using the poster wavelength, you than stack use the absorbance to predict the concentration of unknown tubes. My speculation on the concentration of unknown 2 and unknown 4 were somewhat thirty mg/ml and around disco biscuit mg/ml. (I did not do the unknown number 1, 3, and the actually unknown.

I took the answers from my classmate group) Method First, I determined the intentness of CoCl2 by measuring the absorbance of light at different wavelengths. Second, I constructed a standard curve for cobalt chloride; and then implant expose the equation of absorption spectrum. Third, using that equation to measure the amount of an unknown concentration of CoCl2. For this expe riment, we could have seven hulky test tub! es, 8 small test tubes; three cone-shaped tubes containing CoCl2 solution, DI water supply, and pipettes. For large tubes, each filled with different concentration of CoCl2 (0, 0.1ml, 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml) plus distilled water (10, 9.9ml, 9.0ml, 8.0ml, 7.0ml, 6.0ml, 5.0ml), and labeled form 0 to 6. The offshoot tube was containing only if distilled water (10ml) acts as a...If you want to get at a beneficial essay, order it on our website: OrderCustomPaper.com

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